| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHAFFER, Benny | JR | Mount Union |
56.81 | Mount Union Last Chance Outdoor |
| 2 | MOMEYER, Josh | SO | Mount Union |
55.38 | 2023 Mount Union John Homon Open |
| 3 | DEAS, Dontae | SR | Kent State |
54.80 | Northeast Ohio Open |
| 4 | DAVIS II, Shakorie | SR | Kent State |
54.08 | Northeast Ohio Open |
| 5 | KUPFNER, Aaron | SO | West Virginia Wesleyan |
54.28 | Vertklasse |
| 6 | COLEMAN, Brody | SO | Mount Union |
55.10 | |
| 7 | DESHUK, Dominic | SO | Mount Union |
55.79 | Mount Union Last Chance Outdoor |
| 8 | WEISNER, Conrad | SO | Mount Union |
57.31 | 2022 OAC Outdoor Track and Field Championship |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FARQUHARSON, Reese | FR | West Virginia Wesleyan |
1:04.54 | Vertklasse |
| 2 | BUTLER, Huntyr | FR | Rio Grande |
1:00.00 | |
| 3 | BILAL, Mohamed | SO | Wright State |
58.32 | Golden Grizzly T&F Invite |
| 4 | CHEATHAM, Marshall | FR | West Liberty |
57.53 | Muskingum Invitational |
| 5 | JERRET, Jacob | FR | Mount Union |
58.04 | 2023 Mount Union John Homon Open |
| 6 | NEWBY, Jacob | FR | Mount Union |
58.85 | 2023 Mount Union John Homon Open |
| 7 | HOLSTEIN, Dryden | SO | West Virginia Wesleyan |
1:01.57 | Mountain East Conference Track & Field Championships |
| 8 | CARTER, Caleb | FR | Indiana East |
NT |