| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | West Windsor-Plainsboro South | A | Mankavil | Devpura | Gobo | Tsai | 22:00.00 | |
| 2 | West Windsor-Plainsboro North | A | Arastu | Lee | Sankar | Chinchalkar | 22:30.00 | |
| 3 | Lawrenceville | A | Malik | Chafin | Maldacena | Phelan | 22:45.00 | |
| 4 | Robbinsville | A | Daly | White | Chopra | Johnston | 23:20.00 | |
| 5 | Hopewell Valley Central | A | Armendinger | Dumont | Maclean | Mayer | 23:40.00 | |
| 6 | Hamilton East - Steinert | A | Seeley | Torres | Yilmaz | Zamichieli | NT | |
| 7 | Notre Dame | A | Peregrin | Becker | Bailey | Lawrence | 28:00.00 | |
| 8 | Allentown | A | Rentner | Ruchelman | Mooney | Wroblewski | NT |