Hand Times
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Catholic |
A | Kreuzwieser | Stefans | Stinson | Trapani | 3:45.00 | |
| 3 | A | McClymont | Burkholder | Horning | Songster | 3:37.62 | York College Twilight Meet | |
| 4 | Franklin & Marshall |
A | Towers | Emrey | Braun | Maglione | 3:36.00 | |
| 5 | Thaddeus Stevens |
A | McCaffrey | Gibson | Sauder | Deveaux | 3:36.68 | Mason-Dixon Track & Field Invitational |
| 6 | Immaculata |
A | McHugh | Speakman-Viggiano | Faia | Crews | 3:37.92 | Atlantic East Conference Track & Field Championships |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | RC - Gloucester CC | A | Bruno | Coleman | Reese | Sanchez | DNS | |
| 2 | Alvernia |
A | Short | Jerome | Loehr | Rodriguez Jr. | 3:31.41 | 2021 Misericordia Twilight |
| 3 | Dickinson |
A | Mercer | Sternick | Erdmann | Collins | 3:28.07 | Hopkins/Loyola Track & Field Invitational |
| 4 | McDaniel |
A | Madsen | Stango | Richardson | Razze | 3:24.18 | Hopkins Loyola Invitational |
| 5 | Elizabethtown |
A | Baxter | Schwab | Elliott | Reice | 3:25.59 | 125th Penn Relays |
| 6 | McDaniel |
B | Huffer | Maxwell | Hubbard | Razze | 3:30.00 | |
| 7 | Elizabethtown |
B | Kardos | Kuhn | Wolf | Schalek | DNS | |
| 8 | Elizabethtown Alumni | A | Gerstenbacher | Angelina | Twist | Pizzirusso | 3:35.00 |