| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | TEM | A | Reick, Dave | Lozinak, John | Baer, Andrew | Cameron, Cody | 3:38.00 | |
| 2 | TEM | B | Yayer, Austin | Cheslock, Luke | Elliott, Mason | Ziolkowski, Chris | 3:44.00 | |
| 3 | PSU | A | McElwain, Brody | Kennedy, David | Walwro, Mike | Clark, Ryan | 3:50.00 | |
| 4 | Pitt | B | McFoy, Noah | Freeman, Alec | Cardamone, Nicholas | Case, Zach | 3:59.00 | |
| 5 | PSU | B | Davis, Sean | Theoclitus, Sean | Day, Stephen | Featherstone, Mark | 4:00.00 | |
| 6 | Pitt | A | O'Brien, Steve | Conti, Evan | Beeman, Denzel | Lu, Gordon | 4:00.00 | |
| 7 | GW | A | Connolly, Jack | Lane, Thomas | Gonzalez, Alec | Guzewich, Ellis | 4:10.00 | |
| 8 | NOVA | A | Timm, Brandon | Ross, Liem | Power, Patrick | Willis, Mark | 4:16.00 | |
| 9 | PSU | C | Perdue, Tyler | Verna, Jason | Marino, Thomas | Seip, Aaron | 4:20.00 | |
| 10 | DREX | A | Hiller, Jay | Thompson, Dana | Zerbo, Luke | Stec, Brendan | 4:20.00 |