| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | TEM | A | Oyeola, Bisi | Groncki, Cassie | Preston, Drew | Karluah, Regina | 4:05.00 | |
| 2 | TEM | B | Welles, Gabby | Follis, Lauren | Devenney, KatieRose | Waddington, Sarah | 4:10.00 | |
| 3 | Pitt | A | White, Karleigh | Sisson, Caroline | Beck, Hannah | Kantz, Serena | 4:40.00 | |
| 4 | Pitt | B | McHale, Jacqueline | Failor, Gracie | Carow, Enlin | Hornor, Jenn | 4:50.00 | |
| 5 | GW | A | Hartge, Cecilia | Barry, Hannah | Reach, Brenna | Ojibe, Zania | 4:50.00 | |
| 6 | PSU | A | O'Reilly, Meghan | Thornton, Hannah | Person, Lucia | Ebinger, Mackenzie | 5:00.00 |